RT Journal Article T1 Callus culture: A sustainable approach for preserving and enhancing Cholistan’s endangered medicinal plants for the herbal industry A1 Haider, Muhammad Wasim A1 Nafees, Muhammad A1 Ahmad, Ishtiaq A1 Masood, Asad A1 Raza, Muhammad Samsam A1 Haq, Izhar Ul A1 Saeed, Muhammad Ahmad A1 Shabbir, Aqsa A1 Umar Farooq T2 Journal of Horticultural Science & Technology AB Sustainable preservation techniques are necessary due to the rapid depletion of medicinal plants, which are invaluable resources for herbal products. This review focuses on callus culture as a sustainable approach for conserving and enhancing endangered medicinal plants in the Cholistan desert, Pakistan. The Cholistan desert harbours a rich diversity of medicinally important plant species, but agricultural expansion and urbanization threaten their existence. Traditional preservation methods are often tedious and inefficient, emphasizing the need for alternative approaches. Callus culture offers a promising solution for conserving endangered species and enhancing their bioactive compounds. This technique involves the regeneration of plant tissues from explants under controlled conditions. The review discusses the challenges in preserving medicinal plants, the significance of Cholistan’s flora, and the role of callus culture in pharmaceutical industries. It also highlights the importance of research and development in Pakistan to harness the potential of medicinal plants. Furthermore, the review emphasizes the significance of tissue culture techniques in producing bioactive substances and conserving endangered plant species. It discusses the conservation of callus to ensure the sustainable supply of medicinal plant materials to the industry. In summary, the review highlights the significance of callus culture for the sustainable growth of the herbal sector in Cholistan’s endangered medicinal plants. FD 31 December 2023 YR 2023 DO https://doi.org/10.46653/jhst23064041 VO 6 IS 4 SP 41 OP 49 JO J. Hortic. Sci. Technol. LA English SN 2664-9489 UL https://www.pshsciences.org/publications/jhst/issues/6-4/2023-041/